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J Clin Microbiol. 1975 January; 1(1): 96-101

Purification of Japanese encephalitis virus vaccine by zonal centrifugation.

K Okuda, K Ito, K Miyake, M Morita and M Ogonuki

ABSTRACT

A large-scale procedure for purification and concentration of Japanese encephalitis virus vaccine by a continuous-flow isopycnic banding technique in a sucrose density gradient solution, using a K-III zonal centrifuge rotor, is presented. The quality of zonal-purified vaccine was compared with commercial and Japanese National Institutes of Health reference vaccines for antigenicity, immunodiffusion, and allergic encephalitogenicity tests to show its high purity.

J Clin Microbiol. 1975 January; 1(1): 96-101






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1975 by the American Society for Microbiology. All rights reserved.