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J Clin Microbiol. 1980 January; 11(1): 19-21

Liquid medium for growth of Legionella pneumophila.

J D Ristroph, K W Hedlund and R G Allen

ABSTRACT

The medium described is a simple yeast extract broth capable of growing large number of Legionella neumophila, the causative organism of Legionnaires disease. Filtration was chosen as a means of sterilization, since medium that was autoclaved did not support growth without the presence of Norite A. The filtered medium gave rapid cell growth and maintained the initial antigen production. The observed generation time was 99 min with a maximum cell population of 2 X 10(2) COLONY-FORMING UNITS PER ML IN APPROXIMATELY 40 H.

J Clin Microbiol. 1980 January; 11(1): 19-21




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Copyright © 1980 by the American Society for Microbiology. All rights reserved.