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J Clin Microbiol. 1980 February; 11(2): 132-134

Some characteristics of a new multiple-channel photometer for through-the-plate reading of microplates to be used in enzyme-linked immunosorbent assay.

E J Ruitenberg, V M Sekhuis and B J Brosi

ABSTRACT

The performance of a prototype of a now commercially available multiple-channel photometer for high-speed reading of microplates to be used in the enzyme-linked immunosorbent assay was evaluated. For this purpose the extinction of a stable test liquid dispensed in flat-bottomed polystyrene microplates was measured, varying the test volume and the optical density. From the results obtained for dilutions of a single, biolgoically inactive material we concluded that the photometric inaccuracy is negligible when performing enzyme-linked immunosorbent assay with biologically active samples.

J Clin Microbiol. 1980 February; 11(2): 132-134






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