This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Doskeland, S O Articles by Berdal, B P Search for Related Content PubMed PubMed Citation Articles by Doskeland, S O Articles by Berdal, B P

 Previous Article  |  Next Article 

J Clin Microbiol. 1980 April; 11(4): 380-384

Bacterial antigen detection in body fluids: methods for rapid antigen concentration and reduction of nonspecific reactions.

ABSTRACT

We sought procedures which would allow a rapid concentration in high yield of bacterial antigens from tissue fluids of patients and which could be applied also to protein-rich fluids like serum. Ethanol precipitation at a subzero temperature with albumin added as an antigen coprecipitant made it possible to achieve a more than 20-fold concentration of antigen in 15 min and a 200-fold concentration in 45 min. Heat-stable antigens could be concentrated from protein-rich fluids (like serum) after the sample had been deproteinized by boiling. Such heating (100 degrees C, 3 min) also liberated bacterial polysaccharides from antibody complexes and elminated the nonspecific interference of serum in enzyme-linked immunosorbent assay.

This article has been cited by other articles:

• Blanco, S., Prat, C., Pallares, M. A., Matas, L., Dominguez, J. (2004). Centrifugal Ultrafiltration Method for Rapid Concentration of Legionella pneumophila Urinary Antigen. J. Clin. Microbiol. 42: 4410-4410 [Full Text]  
• Dominguez, J., Blanco, S., Rodrigo, C., Azuara, M., Gali, N., Mainou, A., Esteve, A., Castellvi, A., Prat, C., Matas, L., Ausina, V. (2003). Usefulness of Urinary Antigen Detection by an Immunochromatographic Test for Diagnosis of Pneumococcal Pneumonia in Children. J. Clin. Microbiol. 41: 2161-2163 [Abstract] [Full Text]  
• ELLIS, R. W., SOBANSKI, M. A. (2000). Diagnostic particle agglutination using ultrasound: a new technology to rejuvenate old microbiological methods. J Med Microbiol 49: 853-859 [Abstract] [Full Text]  
• Ravinder, P. T., Parija, S. C., Rao, K. S. V. K. S. (2000). Urinary Hydatid Antigen Detection by Coagglutination, a Cost-Effective and Rapid Test for Diagnosis of Cystic Echinococcosis in a Rural or Field Setting. J. Clin. Microbiol. 38: 2972-2974 [Abstract] [Full Text]  
• Pereira Arias-Bouda, L. M., Nguyen, L. N., Ho, L. M., Kuijper, S., Jansen, H. M., Kolk, A. H. J. (2000). Development of Antigen Detection Assay for Diagnosis of Tuberculosis Using Sputum Samples. J. Clin. Microbiol. 38: 2278-2283 [Abstract] [Full Text]