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J Clin Microbiol. 1984 July; 20(1): 128-130

Microneutralization test in PK(15) cells for assay of antibodies to louping ill virus.

ABSTRACT

A microneutralization test in PK(15) cells was developed to measure the neutralizing antibody response of a group of ponies experimentally challenged with louping ill virus. Viral cytopathic effect was maximal after 6 days of incubation, at which point titration endpoints were clear-cut and readily determinable. The assay compared favorably with the mouse neutralization test for accuracy and ease of performance.

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