This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohno, O.
Right arrow Articles by Lau, J. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohno, O.
Right arrow Articles by Lau, J. Y.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 01 1997, 201-207, Vol 35, No. 1
Copyright © 1997 by the American Society for Microbiology. All rights reserved.

New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a

O Ohno, M Mizokami, RR Wu, MG Saleh, K Ohba, E Orito, M Mukaide, R Williams and JY Lau
Department of Public Health, Nagoya City University Medical School, Japan.

Recent studies have focused on whether different hepatitis C virus (HCV) genotypes are associated with different profiles of pathogenicity, infectivity, and response to antiviral therapy. The establishment of a simple and precise genotyping system for HCV is essential to address these issues. A new genotyping system based on PCR of the core region with genotype-specific PCR primers for the determination of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a was developed. A total of 607 samples (379 from Japan, 63 from the United States, 53 from Korea, 35 from Taiwan, 32 from China, 20 from Hong Kong, 15 from Australia, 6 from Egypt, 3 from Bangladesh, and 1 from South Africa) were tested by both the assay of Okamoto et al. (H. Okamoto, Y. Sugiyama, S. Okada, K. Kurai, Y. Akahane, Y. Sugai, T. Tanaka, K. Sato, F. Tsuda, Y. Miyamura, and M. Mayumi, J. Gen. Virol. 73:673-679, 1992) and this new genotyping system. Comparison of the results showed concordant results for 539 samples (88.8%). Of the 68 samples with discordant results, the nucleotide sequences of the HCV isolates were determined in 23, and their genotypes were determined by molecular evolutionary analysis. In all 23 samples, the assignment of genotype by our new genotyping system was correct. This genotyping system may be useful for large-scale determination of HCV genotypes in clinical studies.


This article has been cited by other articles:

  • Tanaka, Y., Hanada, K., Hanabusa, H., Kurbanov, F., Gojobori, T., Mizokami, M. (2007). Increasing genetic diversity of hepatitis C virus in haemophiliacs with human immunodeficiency virus coinfection. J. Gen. Virol. 88: 2513-2519 [Abstract] [Full Text]  
  • Suruki, R. Y., Mueller, N., Hayashi, K., Harn, D., DeGruttola, V., Raker, C. A., Tsubouchi, H., Stuver, S. O. (2006). Host Immune Status and Incidence of Hepatocellular Carcinoma among Subjects Infected with Hepatitis C Virus: A Nested Case-Control Study in Japan. Cancer Epidemiol. Biomarkers Prev. 15: 2521-2525 [Abstract] [Full Text]  
  • Cook, L., Sullivan, K., Krantz, E. M., Bagabag, A., Jerome, K. R. (2006). Multiplex Real-Time Reverse Transcription-PCR Assay for Determination of Hepatitis C Virus Genotypes. J. Clin. Microbiol. 44: 4149-4156 [Abstract] [Full Text]  
  • Ishii, N., Watashi, K., Hishiki, T., Goto, K., Inoue, D., Hijikata, M., Wakita, T., Kato, N., Shimotohno, K. (2006). Diverse Effects of Cyclosporine on Hepatitis C Virus Strain Replication. J. Virol. 80: 4510-4520 [Abstract] [Full Text]  
  • Suzuki, T., Omata, K., Satoh, T., Miyasaka, T., Arai, C., Maeda, M., Matsuno, T., Miyamura, T. (2005). Quantitative Detection of Hepatitis C Virus (HCV) RNA in Saliva and Gingival Crevicular Fluid of HCV-Infected Patients. J. Clin. Microbiol. 43: 4413-4417 [Abstract] [Full Text]  
  • Kim, Y. J., Kim, S.-O., Chung, H. J., Jee, M. S., Kim, B. G., Kim, K. M., Yoon, J.-H., Lee, H.-S., Kim, C. Y., Kim, S., Yoo, W., Hong, S. P. (2005). Population Genotyping of Hepatitis C Virus by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Short DNA Fragments. Clin. Chem. 51: 1123-1131 [Abstract] [Full Text]  
  • Kremer, J. R., Fack, F., Olinger, C. M., Mulders, M. N., Muller, C. P. (2004). Measles Virus Genotyping by Nucleotide-Specific Multiplex PCR. J. Clin. Microbiol. 42: 3017-3022 [Abstract] [Full Text]  
  • Colina, R., Casane, D., Vasquez, S., Garcia-Aguirre, L., Chunga, A., Romero, H., Khan, B., Cristina, J. (2004). Evidence of intratypic recombination in natural populations of hepatitis C virus. J. Gen. Virol. 85: 31-37 [Abstract] [Full Text]  
  • Corbet, S., Bukh, J., Heinsen, A., Fomsgaard, A. (2003). Hepatitis C Virus Subtyping by a Core-Envelope 1-Based Reverse Transcriptase PCR Assay with Sequencing and Its Use in Determining Subtype Distribution among Danish Patients. J. Clin. Microbiol. 41: 1091-1100 [Abstract] [Full Text]  
  • Visona, K., Baez, F., Taylor, L., Berrios, R., Leon, B., Pacheco, C., Jiron, R., Luftig, R. B., Somarriba, M. M. (2002). Impact of Hepatitis B and Hepatitis C Virus Infections in a Hematology-Oncology Unit at a Children's Hospital in Nicaragua, 1997 to 1999. CVI 9: 622-626 [Abstract] [Full Text]  
  • Kalinina, O., Norder, H., Mukomolov, S., Magnius, L. O. (2002). A Natural Intergenotypic Recombinant of Hepatitis C Virus Identified in St. Petersburg. J. Virol. 76: 4034-4043 [Abstract] [Full Text]  
  • Nakai, K., Win, K. M., Oo, S. S., Arakawa, Y., Abe, K. (2001). Molecular Characteristic-Based Epidemiology of Hepatitis B, C, and E Viruses and GB Virus C/Hepatitis G Virus in Myanmar. J. Clin. Microbiol. 39: 1536-1539 [Abstract] [Full Text]  
  • Tokita, H., Kaufmann, G. R., Matsubayashi, M., Okuda, I., Tanaka, T., Harada, H., Mukaide, M., Suzuki, K., Cooper, D. A. (2000). Hepatitis C Virus Core Mutations Reduce the Sensitivity of a Fluorescence Enzyme Immunoassay. J. Clin. Microbiol. 38: 3450-3452 [Abstract] [Full Text]  
  • Sreevatsan, S., Bookout, J. B., Ringpis, F. M., Pottathil, M. R., Marshall, D. J., De Arruda, M., Murvine, C., Fors, L., Pottathil, R. M., Barathur, R. R. (1998). Algorithmic Approach to High-Throughput Molecular Screening for Alpha Interferon-Resistant Genotypes in Hepatitis C Patients. J. Clin. Microbiol. 36: 1895-1901 [Abstract] [Full Text]  
  • Tang, Y.-W., Procop, G. W., Persing, D. H. (1997). Molecular diagnostics of infectious diseases. Clin. Chem. 43: 2021-2038 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»