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Journal of Clinical Microbiology, January 1998, p. 286-289, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Six Sets of PCR Primers from Two Different Genomic Regions for Amplification of GB Virus C/Hepatitis G Virus RNA

Anton Andonov,* Connie Sauder, Heather Jacobsen, and Rabindra Chaudhary

Laboratory for Viral Hepatitis, Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario, Canada

Received 16 May 1997/Returned for modification 19 August 1997/Accepted 10 October 1997

Forty-four clinical samples positive for GB virus C (GBV-C)/hepatitis G virus (HGV) were tested with six primer sets, four from the 5' untranslated region (5'-UTR) and two from the NS5a genomic region. Two of the 5'-UTR primer sets, when used in a single-round 60-cycle PCR, detected between 86.4 and 97.7% of the positive samples, while two different sets from the same area, when used in a nested PCR, amplified between 97.7 and 100% of the positive specimens. Both sets from the NS5a region, when used in a single-round PCR, detected 95.5% of the GBV-C/HGV-positive samples. Parallel testing with two PCR sets, one from the 5'-UTR and a second from NS5a, may eliminate false-negative results attributable to the genetic heterogeneity of the virus.

* Corresponding author. Mailing address: Laboratory for Viral Hepatitis, Bureau of Microbiology, Laboratory Centre for Disease Control, Virus Bldg. 10, Rm. B-7, Postal Locator 1000E1, Tunney's Pasture, Ottawa, Ontario K1A 0L2, Canada. Phone: (613) 957-0175. Fax: (613) 954-0207. E-mail: Anton_Andonov{at}inet.hwc.ca.

Journal of Clinical Microbiology, January 1998, p. 286-289, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1998 by the American Society for Microbiology. All rights reserved.