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Journal of Clinical Microbiology, January 1998, p. 294-295, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Premarket Evaluation of a Commercial Glycoprotein G-Based Enzyme Immunoassay for Herpes Simplex Virus Type-Specific Antibodies

Rhoda L. Ashley,1,* Linxian Wu,2 Jerry W. Pickering,2 Ming-Chieh Tu,2 and Laura Schnorenberg1

Department of Laboratory Medicine, University of Washington, Seattle, Washington,1 and Gull Laboratories, Inc., Salt Lake City, Utah2

Received 19 June 1997/Returned for modification 18 August 1997/Accepted 8 October 1997

A new commercial glycoprotein G-based enzyme immunoassay (gG-EIA) was compared with Western blotting (WB) for detection of herpes simplex virus type 1 (HSV-1) or HSV-2 type-specific antibodies in 193 serum samples. Sensitivity for HSV-1 was 95%; specificity was 96%. Sensitivity for HSV-2 was 98%; specificity was 97%. Twelve of 13 serum samples with equivocal gG-EIA results were serotyped by WB.

* Corresponding author. Mailing address: Virology Office, G815, Children's Hospital and Medical Center, CH-82, 4800 Sand Point Way, NE, Seattle, WA 98105. Phone: (206) 526-2117. Fax: (206) 527-3885. E-mail: rashley{at}u.washington.edu.

Journal of Clinical Microbiology, January 1998, p. 294-295, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1998 by the American Society for Microbiology. All rights reserved.