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Journal of Clinical Microbiology, January 1998, p. 317-320, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of a Gastric Juice-Based PCR Assay To Detect Helicobacter pylori Infection in Culture-Negative Patients

Haruhiko Yoshida,1,* Katsutaro Hirota,2 Yasushi Shiratori,1 Takeshi Nihei,2 Shin Amano,2 Akira Yoshida,3 Osamu Kawamata,3 and Masao Omata1

Second Department of Internal Medicine, University of Tokyo, Tokyo 113,1 Department of Internal Medicine, Mito Saiseikai Hospital, Ibaraki 311-41,2 and Center for Molecular Biology and Cytogenetics, SRL Inc., Tokyo 192,3 Japan

Received 16 June 1997/Returned for modification 10 September 1997/Accepted 20 October 1997

A gastric juice-based PCR assay was compared with culture, microscopy, and a rapid urease test with specimens from 114 subjects. The PCR and conventional tests were positive for 76 and 62% of the subjects, respectively. The prevalence of gastroduodenal disease and seropositivity for anti-Helicobacter pylori immunoglobulin G were similarly high among conventional-test-positive and PCR-only-positive subjects compared to all-negative ones. The PCR assay is recommended to confirm the H. pylori status of culture-negative peptic-ulcer patients.

* Corresponding author. Mailing address: Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo 113, Japan. Phone: 81-3-3815-5411. Fax: 81-3-3814-0021. E-mail: yoshida-2im{at}h.u-tokyo.ac.jp.

Journal of Clinical Microbiology, January 1998, p. 317-320, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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