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Journal of Clinical Microbiology, February 1998, p. 548-551, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Approaches to Diagnosis of Pulmonary Diseases Due to Mycoplasma pneumoniae

Marianne Abele-Horn,1,* Ulrich Busch,1 Hans Nitschko,1 Enno Jacobs,2 Ralph Bax,3 Friederike Pfaff,1 Beatrix Schaffer,1 and Jürgen Heesemann1

Max von Pettenkofer-Institute, Ludwig-Maximilians-University, 80336 Munich,1 Institute for Microbiology, Technical University, 01307 Dresden,2 and Childrens Hospital Munich-Schwabing, Technical University, 80404 Munich,3 Germany

Received 12 May 1997/Returned for modification 5 August 1997/Accepted 4 November 1997

In this prospective study, the use of a culture-enhanced PCR assay for the detection of Mycoplasma pneumoniae, followed by hybridization with a specific probe (MP-HPCR) or without hybridization (MP-PCR), and the use of a nested PCR (MP-NPCR) were evaluated. Clinical samples (190 specimens) from 190 patients with respiratory complaints were incubated in culture broth overnight and then subjected to PCR. The results of the PCR were compared to those obtained by culture, the direct antigen test, and serologic testing by microparticle agglutination and by immunoblotting in unclear cases. The sensitivities were 19 CFU for MP-PCR, 1.9 CFU for MP-HPCR, and 0.019 CFU for MP-NPCR. PCR amplification of the beta -globin gene was possible in 98% of cases: after dilution of the beta -globin-negative samples, all samples were reactive. Correlation between negative MP-NPCR results and negative serology results was found in 89% of cases; a positive correlation was found with 10% of the patients. Samples from three immunocompromised patients were MP-NPCR positive but serologically negative. High respiratory colonization by M. pneumoniae (>105 CFU/ml) in patients with acute respiratory disease could be detected by culture, MP-PCR, and MP-NPCR. These results indicate that MP-PCR and MP-NPCR are reliable methods for the detection of M. pneumoniae in respiratory tract samples of patients with respiratory complaints.

* Corresponding author. Mailing address: Max von Pettenkofer-Institute, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, 80336 Munich, Germany. Phone: 49-89-5160-5245. Fax: 49-89-64 270 288. E-mail: 08964270286-0001{at}t-online.de.

Journal of Clinical Microbiology, February 1998, p. 548-551, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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