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Journal of Clinical Microbiology, May 2002, p. 1651-1655, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1651-1655.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Mycobacterium tuberculosis Complex Isolates from the Cerebrospinal Fluid of Meningitis Patients at Six Fever Hospitals in Egypt

Robert C. Cooksey,^1* Said H. Abbadi,^1, Charles L. Woodley,¹ David Sikes,¹ Momtaz Wasfy,² Jack T. Crawford,¹ and Frank Mahoney²

Division of AIDS, STD, and TB Laboratory Research, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,¹ U.S. Naval Medical Research Unit No. 3, Cairo, Egypt²

Received 10 December 2001/ Returned for modification 27 January 2002/ Accepted 5 February 2002

Mycobacterium tuberculosis complex isolates from cerebrospinal fluid of 67 meningitis patients were obtained from six fever hospitals in Egypt. One M. bovis and 66 M. tuberculosis isolates were identified by PCR-restriction fragment length polymorphism (RFLP) analysis of oxyR. Among the M. tuberculosis isolates, 53 unique strain types (with 3 to 16 copies of IS6110) were found by RFLP analyses. Nine clusters (eight with two isolates each and one with six isolates) were also found. Thirty-six spoligotypes, including at least 10 that have been previously reported from other countries, were also observed. Forty-one (62.1%) of the isolates were in spoligotype clusters, and 22 (33%) of the isolates were in RFLP clusters. Fifty-one of the isolates were susceptible in vitro to all of the antituberculosis drugs tested, 11 were monoresistant to capreomycin, rifampin, isoniazid (INH), pyrazinamide, or streptomycin (STR), 4 were resistant to STR and INH, and 1 was resistant to STR, INH, and ethambutol.

Present address: Department of Microbiology, Suez Canal University School of Medicine, Ismailia, Egypt.

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