This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shu, P.-Y. Articles by Huang, J.-H. Search for Related Content PubMed PubMed Citation Articles by Shu, P.-Y. Articles by Huang, J.-H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2002, p. 1840-1844, Vol. 40, No. 5
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.5.1840-1844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Potential Application of Nonstructural Protein NS1 Serotype-Specific Immunoglobulin G Enzyme-Linked Immunosorbent Assay in the Seroepidemiologic Study of Dengue Virus Infection: Correlation of Results with Those of the Plaque Reduction Neutralization Test

Division of Research and Diagnosis, Center for Disease Control, Department of Health,¹ Co-Wealth Medical Science and Biotechnology, Taipei,³ Department of Emergency Medicine, Medical Center, Tzu Chi University, Hualien, Taiwan, Republic of China²

Received 16 August 2001/ Returned for modification 20 October 2001/ Accepted 17 February 2002

An NS1 serotype-specific indirect enzyme-linked immunosorbent assay (ELISA) was developed to differentiate primary and secondary dengue virus infections and serotypes of primary dengue virus infection. For this report, we carried out retrospective seroepidemiologic studies on serum samples collected from residents of Liuchiu Hsiang, Pingtung County, an isolated island in southern Taiwan during 1997-1998. The results demonstrated that good correlation existed between dengue virus NS1 serotype-specific immunoglobulin G (IgG) ELISA and dengue virus plaque reduction neutralization test (PRNT). Our data suggested that NS1 serotype-specific IgG ELISA could replace PRNT for seroepidemiologic studies to differentiate Japanese encephalitis and dengue virus infections and for dengue virus serotyping.

This article has been cited by other articles:

• Qiu, L.-w., Di, B., Wen, K., Wang, X.-s., Liang, W.-h., Wang, Y.-d., Pan, Y.-x., Wang, M., Ding, Y.-q., Che, X.-y. (2009). Development of an Antigen Capture Immunoassay Based on Monoclonal Antibodies Specific for Dengue Virus Serotype 2 Nonstructural Protein 1 for Early and Rapid Identification of Dengue Virus Serotype 2 Infections. CVI 16: 88-95 [Abstract] [Full Text]  
• Lee, I.-K., Liu, J.-W., Yang, K. D. (2008). Clinical and Laboratory Characteristics and Risk Factors for Fatality in Elderly Patients with Dengue Hemorrhagic Fever. Am J Trop Med Hyg 79: 149-153 [Abstract] [Full Text]  
• Dussart, P., Labeau, B., Lagathu, G., Louis, P., Nunes, M. R. T., Rodrigues, S. G., Storck-Herrmann, C., Cesaire, R., Morvan, J., Flamand, M., Baril, L. (2006). Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum. CVI 13: 1185-1189 [Abstract] [Full Text]  
• Xu, H., Di, B., Pan, Y.-x., Qiu, L.-w., Wang, Y.-d., Hao, W., He, L.-j., Yuen, K.-y., Che, X.-y. (2006). Serotype 1-Specific Monoclonal Antibody-Based Antigen Capture Immunoassay for Detection of Circulating Nonstructural Protein NS1: Implications for Early Diagnosis and Serotyping of Dengue Virus Infections.. J. Clin. Microbiol. 44: 2872-2878 [Abstract] [Full Text]  
• KHOR, B.-S., LIU, J.-W., LEE, I.-K., YANG, K. D. (2006). Dengue hemorrhagic Fever patients with acute abdomen: clinical experience of 14 cases.. Am J Trop Med Hyg 74: 901-904 [Abstract] [Full Text]  
• LEE, I.-K., LIU, J.-W., YANG, K. D. (2005). CLINICAL CHARACTERISTICS AND RISK FACTORS FOR CONCURRENT BACTEREMIA IN ADULTS WITH DENGUE HEMORRHAGIC FEVER. Am J Trop Med Hyg 72: 221-226 [Abstract] [Full Text]  
• Shu, P.-Y., Huang, J.-H. (2004). Current Advances in Dengue Diagnosis. CVI 11: 642-650 [Full Text]  
• Shu, P.-Y., Chen, L.-K., Chang, S.-F., Su, C.-L., Chien, L.-J., Chin, C., Lin, T.-H., Huang, J.-H. (2004). Dengue Virus Serotyping Based on Envelope and Membrane and Nonstructural Protein NS1 Serotype-Specific Capture Immunoglobulin M Enzyme-Linked Immunosorbent Assays. J. Clin. Microbiol. 42: 2489-2494 [Abstract] [Full Text]  
• Wong, S. J., Boyle, R. H., Demarest, V. L., Woodmansee, A. N., Kramer, L. D., Li, H., Drebot, M., Koski, R. A., Fikrig, E., Martin, D. A., Shi, P.-Y. (2003). Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination. J. Clin. Microbiol. 41: 4217-4223 [Abstract] [Full Text]  
• Shu, P.-Y., Chen, L.-K., Chang, S.-F., Yueh, Y.-Y., Chow, L., Chien, L.-J., Chin, C., Lin, T.-H., Huang, J.-H. (2003). Comparison of Capture Immunoglobulin M (IgM) and IgG Enzyme-Linked Immunosorbent Assay (ELISA) and Nonstructural Protein NS1 Serotype-Specific IgG ELISA for Differentiation of Primary and Secondary Dengue Virus Infections. CVI 10: 622-630 [Abstract] [Full Text]