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Journal of Clinical Microbiology, September 2002, p. 3167-3171, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3167-3171.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Simian Immunodeficiency Virus in Diverse Species and of Human Immunodeficiency Virus Type 2 by Using Consensus Primers within the pol Region

Silvina Masciotra,1 Chunfu Yang,1 Danuta Pieniazek,2 Chanda Thomas,1 Sherry M. Owen,1 Harold M. McClure,3 and Renu B. Lal1*

HIV Immunology and Diagnostics Branch,1 HIV and Retrovirology Branch, Division of AIDS, Sexually Transmitted Diseases, and Tuberculosis Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,2 Yerkes Primate Research Center, Emory University, Atlanta, Georgia 303223

Received 13 March 2002/ Accepted 18 May 2002

Human immunodeficiency virus type 2 (HIV-2) is the result of cross-species transmission of simian immunodeficiency virus (SIV) from sooty mangabey monkeys to humans. Primer pairs (intHIV-2/SIV) based on a region of integrase that has considerable homology across HIV-2 and SIV lineages were designed to develop a broadly cross-reactive molecular assay to detect lentivirus infection in primates. The intHIV-2/SIV primers detect HIV-2 and simian viruses SIVcpz, SIVsmm, SIVsyk, SIVagm, and SIVmnd. The primers are also capable of amplifying some HIV-1 strains. Additionally, sequences from the integrase amplicons were of sufficient genetic diversity to permit not only phylogenetic clustering of all simian viruses to their respective lineages but also HIV type and group classification. Thus, the primers described here provide a method to detect primate lentiviruses from diverse species of nonhuman primates, as well as from persons infected with HIV-1 and HIV-2.

* Corresponding author. Mailing address: HIV Immunology and Diagnostics Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-1036. Fax: (404) 639-2660. E-mail: rbl3{at}cdc.gov.

Journal of Clinical Microbiology, September 2002, p. 3167-3171, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3167-3171.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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