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Journal of Clinical Microbiology, December 2003, p. 5429-5433, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5429-5433.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Changes in Gene Cassettes of Class 1 Integrons among Escherichia coli Isolates from Urine Specimens Collected in Korea during the Last Two Decades

Hak Sun Yu,1 Je Chul Lee,2 Hee Young Kang,1 Dong Woo Ro,1 Jae Young Chung,1 Young Sook Jeong,1 Seong Ho Tae,1 Chul Hee Choi,1 Eun Young Lee,1 Sung Yong Seol,1 Yoo Chul Lee,1 and Dong Taek Cho1*

Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, 700-422,1 Department of Microbiology, Eulji University School of Medicine, Daejeon, 301-832, Korea2

Received 30 June 2003/ Returned for modification 15 August 2003/ Accepted 8 September 2003

Gene cassettes of class 1 integrons in Escherichia coli isolates from urine specimens collected in Korea during the last 2 decades were characterized. intI1 was detected in 54% of the isolates, yet gene cassette regions were amplified in only 43% of the isolates. intI2 was detected in 29 (5%) isolates, and no intI3 was detected in this study. Twenty-one different genes, including genes encoding resistance to antibiotics, an alcohol dehydrogenase gene (adhE), and unknown genes, were detected. The genes most commonly found in class 1 integrons were those for aminoglycoside and trimethoprim resistance. The occurrence of aminoglycoside resistance genes in class 1 integrons decreased, and the presence of dfr genes increased rapidly, during the last 2 decades. Single-gene cassettes were predominant during the 1980s, while multigene cassettes predominated from the 1990s on. The aadA1, aadA2, and blaP1-aadA2 gene cassettes were frequently found in isolates from the 1980s but were not detected in isolates recovered since 2000. dfrA12-aadA2 and dfrA17-aadA5 were the most prevalent gene cassettes among isolates recovered from the 1990s on. In conclusion, class 1 integrons would appear to be responsible for resistance to antibiotics commonly used to treat urinary tract infections, and selection of a specific gene cassette was found to occur over the course of time.

* Corresponding author. Mailing address: Department of Microbiology, Kyungpook National University School of Medicine, 101 Dongin-dong, Chung-gu, Daegu, 700-422, Korea. Phone: 82-53-420-6951. Fax: 82-53-427-5664. E-mail: dtcho{at}knu.ac.kr.

Journal of Clinical Microbiology, December 2003, p. 5429-5433, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5429-5433.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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