This Article Full Text Full Text (PDF) Supplemental material Other Versions of this Article: JCM.01870-06v1 45/2/443    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lin, B. Articles by Stenger, D. A. Search for Related Content PubMed PubMed Citation Articles by Lin, B. Articles by Stenger, D. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2007, p. 443-452, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01870-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Using a Resequencing Microarray as a Multiple Respiratory Pathogen Detection Assay ^,

Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375,¹ NOVA Research Incorporated, Alexandria, Virginia 22308,² Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, California 92186³

Received 8 September 2006/ Returned for modification 27 October 2006/ Accepted 15 November 2006

Simultaneous testing for detection of infectious pathogens that cause similar symptoms (e.g., acute respiratory infections) is invaluable for patient treatment, outbreak prevention, and efficient use of antibiotic and antiviral agents. In addition, such testing may provide information regarding possible coinfections or induced secondary infections, such as virally induced bacterial infections. Furthermore, in many cases, detection of a pathogen requires more than genus/species-level resolution, since harmful agents (e.g., avian influenza virus) are grouped with other, relatively benign common agents, and for every pathogen, finer resolution is useful to allow tracking of the location and nature of mutations leading to strain variations. In this study, a previously developed resequencing microarray that has been demonstrated to have these capabilities was further developed to provide individual detection sensitivity ranging from 10¹ to 10³ genomic copies for more than 26 respiratory pathogens while still retaining the ability to detect and differentiate between close genetic neighbors. In addition, the study demonstrated that this system allows unambiguous and reproducible sequence-based strain identification of the mixed pathogens. Successful proof-of-concept experiments using clinical specimens show that this approach is potentially very useful for both diagnostics and epidemic surveillance.

Published ahead of print on 29 November 2006.

Supplemental material for this article may be found at http://jcm.asm.org/.

This article has been cited by other articles:

• Malanoski, A. P., Lin, B., Stenger, D. A. (2008). A model of base-call resolution on broad-spectrum pathogen detection resequencing DNA microarrays. Nucleic Acids Res 36: 3194-3201 [Abstract] [Full Text]  
• Miller, S. M., Tourlousse, D. M., Stedtfeld, R. D., Baushke, S. W., Herzog, A. B., Wick, L. M., Rouillard, J. M., Gulari, E., Tiedje, J. M., Hashsham, S. A. (2008). In Situ-Synthesized Virulence and Marker Gene Biochip for Detection of Bacterial Pathogens in Water. Appl. Environ. Microbiol. 74: 2200-2209 [Abstract] [Full Text]  
• Blyn, L. B., Hall, T. A., Libby, B., Ranken, R., Sampath, R., Rudnick, K., Moradi, E., Desai, A., Metzgar, D., Russell, K. L., Freed, N. E., Balansay, M., Broderick, M. P., Osuna, M. A., Hofstadler, S. A., Ecker, D. J. (2008). Rapid Detection and Molecular Serotyping of Adenovirus by Use of PCR Followed by Electrospray Ionization Mass Spectrometry. J. Clin. Microbiol. 46: 644-651 [Abstract] [Full Text]  
• Lever, A., Mackenzie, I. (2007). Sepsis: definition, epidemiology, and diagnosis. BMJ 335: 879-883 [Full Text]  
• Quan, P.-L., Palacios, G., Jabado, O. J., Conlan, S., Hirschberg, D. L., Pozo, F., Jack, P. J. M., Cisterna, D., Renwick, N., Hui, J., Drysdale, A., Amos-Ritchie, R., Baumeister, E., Savy, V., Lager, K. M., Richt, J. A., Boyle, D. B., Garcia-Sastre, A., Casas, I., Perez-Brena, P., Briese, T., Lipkin, W. I. (2007). Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray. J. Clin. Microbiol. 45: 2359-2364 [Abstract] [Full Text]  
• Pingle, M. R., Granger, K., Feinberg, P., Shatsky, R., Sterling, B., Rundell, M., Spitzer, E., Larone, D., Golightly, L., Barany, F. (2007). Multiplexed Identification of Blood-Borne Bacterial Pathogens by Use of a Novel 16S rRNA Gene PCR-Ligase Detection Reaction-Capillary Electrophoresis Assay. J. Clin. Microbiol. 45: 1927-1935 [Abstract] [Full Text]