This Article Full Text Full Text (PDF) Other Versions of this Article: JCM.01871-06v1 45/3/707    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Harrington, S. M. Articles by Murray, P. R. Search for Related Content PubMed PubMed Citation Articles by Harrington, S. M. Articles by Murray, P. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2007, p. 707-714, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01871-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Genotypic Analysis of Invasive Streptococcus pneumoniae from Mali, Africa, by Semiautomated Repetitive-Element PCR and Pulsed-Field Gel Electrophoresis

S. M. Harrington,^1* F. Stock,¹ A. L. Kominski,¹ J. D. Campbell,² J. C. Hormazabal,³ S. Livio,² L. Rao,^1, K. L. Kotloff,² S. O. Sow,⁴ and P. R. Murray¹

Department of Laboratory Medicine, National Institutes of Health, Bethesda, Maryland,¹ Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland,² Microbiology Department, Instituto de Salud Publica, Santiago, Chile;,³ Center for Vaccine Development, Bamako, Mali⁴

Received 8 September 2006/ Returned for modification 26 October 2006/ Accepted 13 December 2006

As part of a large, ongoing study of invasive infections in pediatric patients in Bamako, Mali, 106 cases of invasive pneumococcal disease were identified from June 2002 to July 2003 (J. D. Campbell et al., Pediatr. Infect. Dis. J. 23:642-649, 2004). Of the 12 serotypes present, the majority of isolates were not contained in PCV7 (the 7-valent pneumococcal conjugate vaccine), including 1 isolate that was serotype 1, 12 isolates that were serotype 2, 58 isolates that were serotype 5, 7 isolates that were serotype 7F, and 1 isolate that was serotype 12F. To determine whether clonal dissemination of the predominant serotypes had taken place, genotyping was performed on 100 S. pneumoniae isolates by using two methods: pulsed-field gel electrophoresis (PFGE) of SmaI-digested genomic DNA, and the Bacterial Barcodes repetitive-element PCR (rep-PCR) method. Criteria for delineating rep-PCR genotypes were established such that isolates of different serotypes were generally not grouped together. The two methods were equally discriminatory within a given pneumococcal serotype. PFGE separated the isolates into 15 genotypes and 7 subtypes; rep-PCR separated isolates into 15 genotypes and 6 subtypes. Using either method, isolates within serotypes 2, 5, and 7 formed three large, separate clusters containing 1 genotype each. Both methods further distinguished related subtypes within serotypes 2 and 5. Interestingly, one of the PFGE subtypes of serotype 5 is indistinguishable from the Columbia⁵-19 clone circulating in Latin America since 1994. The data support that serotypes 2 and 5 were likely to be the result of dissemination of particular clones, some of which are responsible for invasive disease over a broad population range.

Published ahead of print on 27 December 2006.

Present address: University of Maryland, School of Medicine Veterinary Resources, Baltimore, MD 21201.

This article has been cited by other articles:

• Jacobs, M. R., Bajaksouzian, S., Bonomo, R. A., Good, C. E., Windau, A. R., Hujer, A. M., Massire, C., Melton, R., Blyn, L. B., Ecker, D. J., Sampath, R. (2009). Occurrence, Distribution, and Origins of Streptococcus pneumoniae Serotype 6C, a Recently Recognized Serotype. J. Clin. Microbiol. 47: 64-72 [Abstract] [Full Text]  
• Montero, C. I., Stock, F., Murray, P. R. (2008). Mechanisms of Resistance to Daptomycin in Enterococcus faecium. Antimicrob. Agents Chemother. 52: 1167-1170 [Abstract] [Full Text]