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Journal of Clinical Microbiology, March 2007, p. 858-864, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01900-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Correlation of Neo-Sensitabs Tablet Diffusion Assay Results on Three Different Agar Media with CLSI Broth Microdilution M27-A2 and Disk Diffusion M44-A Results for Testing Susceptibilities of Candida spp. and Cryptococcus neoformans to Amphotericin B, Caspofungin, Fluconazole, Itraconazole, and Voriconazole{triangledown}

A. Espinel-Ingroff,1* E. Canton,2 D. Gibbs,3 and A. Wang3

VCU Medical Center, Richmond, Virginia,1 Hospital La Fe, Valencia, Spain,2 Giles Scientific Inc., Santa Barbara, California3

Received 13 September 2006/ Returned for modification 6 November 2006/ Accepted 26 December 2006

We compared the Neo-Sensitabs tablet assay to both reference M27-A2 broth microdilution and M44-A disk diffusion methods for testing susceptibilities of 110 isolates of Candida spp. and Cryptococcus neoformans to amphotericin B, caspofungin, fluconazole, itraconazole, and voriconazole. Neo-Sensitabs assay inhibition zone diameters in millimeters on three agars (Mueller-Hinton agar supplemented with 2% dextrose and 0.5 µg/ml methylene blue [MGM], Shadomy [SHA], and RPMI 1640 [RPMI, 2% dextrose]) were obtained at 24 to 72 h. The correlation coefficient of Neo-Sensitabs results with MICs was similar to that of the disk method for most of the five agents on MGM (R, 0.80 to 0.89 versus 0.76 to 0.89, respectively). Overall, superior correlation was observed at 24 h for most agents. The exception was amphotericin B (R values of 0.68 and 0.5 for disk and tablet, respectively, at 48 h versus 0.68 and 0.48, respectively, at 24 h). In general, Neo-Sensitabs results were less consistent on SHA and RPMI agars. Although agreement by breakpoint category of Neo-Sensitabs and disk results with CLSI method M27-A2 was also similar on MGM (92.7 to 98.2% versus 95.5 to 100%, respectively), the Neo-Sensitabs method failed to identify two of the six isolates with high amphotericin B MICs. These data suggest the potential value of the Neo-Sensitabs assay for testing at least four of the five agents against yeasts evaluated in the clinical laboratory.

* Corresponding author. Mailing address: Division of Infectious Diseases, VCU Medical Center, 1101 Marshal St., Sanger Hall, Room 7049, Richmond, VA 23298-0049. Phone: (804) 828-5743. Fax: (804) 828-3097. E-mail: avingrof{at}vcu.edu.

{triangledown} Published ahead of print on 10 January 2007.

Journal of Clinical Microbiology, March 2007, p. 858-864, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01900-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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