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Journal of Clinical Microbiology, July 2007, p. 2260-2262, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00848-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms{triangledown}

Alma C. van de Pol,1,2 Anton M. van Loon,1 Tom F. W. Wolfs,2 Nicolaas J. G. Jansen,3 Monique Nijhuis,1 Els Klein Breteler,1 Rob Schuurman,1 and John W. A. Rossen1,4*

Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands,1 Department of Pediatrics, Division of Infectious Disease, Wilhelmina Children's Hospital, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, The Netherlands,2 Department of Pediatrics, Division of Intensive Care, Wilhelmina Children's Hospital, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, The Netherlands,3 Laboratory of Medical Microbiology and Immunology, St. Elisabeth Hospital Tilburg, Hilvarenbeekseweg 60, 5022 GC Tilburg, The Netherlands4

Received 21 April 2007/ Returned for modification 26 April 2007/ Accepted 7 May 2007

Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

* Corresponding author. Mailing address: Laboratory of Medical Microbiology and Immunology, St. Elisabeth Hospital Tilburg, Hilvarenbeekseweg 60, 5022 GC Tilburg, The Netherlands. Phone: 31 13 5392650. Fax: 31 13 5441264. E-mail: j.rossen{at}elisabeth.nl

{triangledown} Published ahead of print on 16 May 2007.

Journal of Clinical Microbiology, July 2007, p. 2260-2262, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00848-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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