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Journal of Clinical Microbiology, July 2007, p. 2309-2311, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.00144-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Performance of the HerpeSelect (Focus) and Kalon Enzyme-Linked Immunosorbent Assays for Detection of Antibodies against Herpes Simplex Virus Type 2 by Use of Monoclonal Antibody-Blocking Enzyme Immunoassay and Clinicovirological Reference Standards in Brazil

Clinical Research Unit, Department of Infectious & Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom,¹ Laboratório de Virologia do Instituto de Medicina Tropical and Departamento de Moléstias Infecciosas e Parasitárias da Faculdade de Medicina, Universidade de São Paulo, Av. Dr Eneas de Carvalho Aguiar, 470, Sao Paulo, Brazil,² Fundação Pró-sangue/Hemocentro de São Paulo, Sao Paulo, Brazil,³ Virus Reference Laboratory, Health Protection Agency, Colindale, London NW9 5EQ, United Kingdom⁴

Received 19 January 2007/ Returned for modification 5 March 2007/ Accepted 5 May 2007

A total of 586 serum samples were used to evaluate the performance of type-specific herpes simplex virus type 2 (HSV-2) commercial enzyme-linked immunosorbent assays (ELISAs) by using the monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and a clinicovirological panel as reference standards. The Kalon and HerpeSelect ELISAs had similar sensitivities (93.5% and 93.8% compared with the results obtained by MAb-EIA, respectively, and 100% for both ELISAs compared with the results obtained with a clinicovirological panel). The Kalon ELISA had a higher specificity (96.5% and 96.8% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively) than the HerpeSelect ELISA (86.9% and 94% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively). A higher cutoff significantly improved the specificity of the HerpeSelect ELISA.

Published ahead of print on 16 May 2007.

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