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Journal of Clinical Microbiology, August 2007, p. 2564-2569, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00679-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Simplified and Reliable Scheme for Species-Level Identification of Staphylococcus Clinical Isolates{triangledown}

Natalia Lopes Pontes Iorio,1 Rosana Barreto Rocha Ferreira,1 Ricardo Pinto Schuenck,1 Karoline Lourenço Malvar,1 Anike Pereira Brilhante,1 Ana Paula Ferreira Nunes,2 Carla Callegário Reis Bastos,3 and Kátia Regina Netto dos Santos1*

Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro,1 Department of Pathology, Federal University of Espírito Santo, Espírito Santo,2 Marcílio Dias Naval Hospital, Rio de Janeiro, Brazil3

Received 27 March 2007/ Returned for modification 18 May 2007/ Accepted 23 May 2007

Reliable and rapid identification of staphylococcal strains continues to be a problem faced by many microbiology laboratories. This study evaluates a simplified method that uses a flowchart to assist in the identification of 12 clinical species of Staphylococcus, including eight subspecies. A total of 198 isolates and 11 control strains were identified by the reference method, which employed 22 tests. The results were compared with those obtained by two other methods: an automated system (MicroScan WalkAway) and a simplified method composed of nine tests. The simplified scheme showed an accuracy of 98.5%, while the automated method showed an accuracy of 79.3% (P < 0.001), in identifying staphylococcal species. Atypical phenotypic profiles were detected by both the reference (55.6%) and the simplified (19.7%) methods. The simplified method proposed here was shown to be reliable, with the advantage of being more practical and economic than the reference method.

* Corresponding author. Mailing address: Laboratório de Infecções Hospitalares, Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes, CCS, Bloco I, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP 21941-590. Phone: 55-21-2260-4193. Fax: 55-21-2560-8028. E-mail: santoskrn{at}micro.ufrj.br

{triangledown} Published ahead of print on 30 May 2007.

Journal of Clinical Microbiology, August 2007, p. 2564-2569, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00679-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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