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Journal of Clinical Microbiology, October 2008, p. 3243-3245, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00083-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid and Inexpensive Detection of Multidrug-Resistant Mycobacterium tuberculosis with the Nitrate Reductase Assay Using Liquid Medium and Direct Application to Sputum Samples{triangledown}

Dissou Affolabi,1,2* Mathieu Odoun,1 N'Dira Sanoussi,1 Anandi Martin,2,3 Juan Carlos Palomino,2 Luc Kestens,4 Séverin Anagonou,1 and Françoise Portaels2

Laboratoire de Référence des Mycobactéries, BP 817 Cotonou, Bénin,1 Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium,2 Médecins Sans Frontières, 75011 Paris, France,3 Immunology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium4

Received 15 January 2008/ Returned for modification 13 April 2008/ Accepted 19 August 2008

For rapid and low-cost detection of multidrug-resistant (MDR) Mycobacterium tuberculosis, we applied the nitrate reductase assay (NRA) using a liquid medium directly to sputum samples. A total of 179 sputum samples were analyzed by the NRA, and results were compared to those obtained by the indirect proportion method (IPM) as a standard reference. Out of 144 specimens for which comparable results were available, only one discrepant result was obtained: MDR by NRA but susceptible by the IPM. In total 56% of the results were obtained in 10 days by the NRA. NRA performed in liquid medium is rapid and inexpensive and can be easily implemented in low-income countries.

* Corresponding author. Mailing address: Laboratoire de Référence des Mycobactéries, 01 BP 817 Cotonou, Bénin. Phone: 229-21-33-15-33. Fax: 229-21-33-70-57. E-mail: affolabi_dissou{at}yahoo.fr

{triangledown} Published ahead of print on 27 August 2008.

Journal of Clinical Microbiology, October 2008, p. 3243-3245, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00083-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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