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Journal of Clinical Microbiology, February 2008, p. 789-791, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.00959-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of the One-Step Multiplex Real-Time Reverse Transcription-PCR ProFlu-1 Assay for Detection of Influenza A and Influenza B Viruses and Respiratory Syncytial Viruses in Children{triangledown}

Jérôme LeGoff,1,2* Rachid Kara,1 Florence Moulin,3 Ali Si-Mohamed,1 Anne Krivine,4 Laurent Bélec,1 and Pierre Lebon4

Laboratoire de Virologie, Hôpital Européen Georges Pompidou, and Université Paris Descartes, Paris, France,1 Laboratoire de Microbiologie, Hôpital Saint Louis, Paris, France,2 Urgences Pédiatriques, Hôpital Cochin-Saint Vincent de Paul, Paris, France,3 Laboratoire de Virologie, Hôpital Cochin-Saint Vincent de Paul, and Université Paris Descartes, Paris, France4

Received 9 May 2007/ Returned for modification 18 July 2007/ Accepted 20 November 2007

We evaluated the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for the detection of influenza A and influenza B viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. As assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the ProFlu-1 assay ranged from 97% to 100%. In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Hôpital Saint Louis, 10 Avenue Claude Vellefaux, Paris 75010, France. Phone: (33)1 42 49 94 84. Fax: (33)1 42 49 92 00. E-mail: jerome.le-goff{at}sls.aphp.fr

{triangledown} Published ahead of print on 5 December 2007.

Journal of Clinical Microbiology, February 2008, p. 789-791, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.00959-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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