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Journal of Clinical Microbiology, June 2008, p. 2088-2091, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00126-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Quality Control Assessment of Human Immunodeficiency Virus Type 2 (HIV-2) Viral Load Quantification Assays: Results from an International Collaboration on HIV-2 Infection in 2006{triangledown}

Florence Damond,1* Antoine Benard,2 Jean Ruelle,3 Abraham Alabi,4 Bernd Kupfer,5 Perpetua Gomes,6 Berta Rodes,7 Jan Albert,8 Jürg Böni,9 Jeremy Garson,10 Bridget Ferns,10 Sophie Matheron,11 Geneviève Chene,2 Françoise Brun-Vezinet,1 for the ACHIEV2E (a Collaboration on HIV-2 Infection) Study Group

APHP, Hôpital Bichat—Claude Bernard, Laboratoire de Virologie, Paris, 75018 France,1 INSERM, U897.ISPED, Université Victor Segalen Bordeaux 2, Bordeaux, F-33076, France,2 AIDS Reference Laboratory, Université Catholique de Louvain, Louvain-la-Neuve, Belgium,3 Medical Research Council Laboratories, Banjul, Gambia,4 Institute of Virology, Bonn, Germany,5 Laboratório de Biologia Molecular, CHLO—Hospital Egas Moniz, Lisboa, Portugal,6 Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain,7 Department of Virology, Swedish Institute for Infectious Disease Control, Solna, and Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden,8 Swiss HIV Cohort Study, Zurich, Switzerland,9 Centre for Virology, Royal Free & University College London Medical School, London, United Kingdom,10 APHP, Hôpital Bichat—Claude Bernard, Service de Maladies Infectieuses et Tropicales, Paris, France,11

Received 22 January 2008/ Accepted 14 April 2008

Human immunodeficiency virus type 2 (HIV-2) RNA quantification assays used in nine laboratories of the ACHIEV2E (A Collaboration on HIV-2 Infection) study group were evaluated. In a blinded experimental design, laboratories quantified three series of aliquots of an HIV-2 subtype A strain, each at a different theoretical viral load. Quantification varied between laboratories, and international standardization of quantification assays is strongly needed.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Bichat—Claude Bernard, Paris, France. Phone: 33 1 40 25 61 51. Fax: 33 1 40 25 67 69. E-mail: florence.damond{at}bch.aphp.fr

{triangledown} Published ahead of print on 23 April 2008.

Journal of Clinical Microbiology, June 2008, p. 2088-2091, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.00126-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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