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Journal of Clinical Microbiology, September 2008, p. 3104-3106, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.01294-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Pan-Dengue Virus Detection by PCR for Travelers Returning from the Tropics

Alexis Dumoulin,¹ Hanspeter Marti,² Marcus Panning,^3, Christoph Hatz,⁴ and Hans H. Hirsch^1,5*

Division of Diagnostics, Institute for Medical Microbiology, Department of Biomedicine, University of Basel, Basel, Switzerland,¹ Department of Diagnostics, Swiss Tropical Institute, Basel, Switzerland,² Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany,³ Medical Department, Swiss Tropical Institute, Basel, Switzerland,⁴ Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland⁵

Received 9 July 2008/ Accepted 11 July 2008

We performed dengue virus (DENV) serology and quantitative real-time pan-DENV reverse transcription-PCR (RT-PCR) on 186 sera of 171 patients returning from the tropics. DENV loads significantly decreased with increasing times of disease and were higher in immunoglobulin M-negative samples. In the first week of disease, pan-DENV RT-PCR is the test of choice.

Published ahead of print on 23 July 2008.

Present address: Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.