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Journal of Clinical Microbiology, September 2008, p. 3144-3146, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00791-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Two Strains of Human Parechovirus 4 Isolated from Two Clinical Cases in Fukuoka City, Japan

Health Science Division, Fukuoka City Institute for Hygiene and the Environment, 2-1-34 Jigyohama, Chuo-ku, Fukuoka-shi, Fukuoka 810-0065, Japan,¹ Virology Section, Niigata Prefectural Institute of Public Health and Environmental Sciences, Niigata, Japan,² Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan³

Received 27 April 2008/ Returned for modification 17 June 2008/ Accepted 11 July 2008

Reverse transcription-PCR targeting the VP0 gene of human parechoviruses (HPeVs) was used to identify two isolates from two Japanese children's stool specimens. Molecular analysis revealed that these isolates belonged to HPeV type 4, and their nucleotide identity in the P1 region was 85.0%.

Published ahead of print on 23 July 2008.