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J Clin Microbiol. 1978 December; 8(6): 643-647

Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae.

D C Yong and A Prytula

ABSTRACT

A rapid carbohydrate utilization procedure for the confirmation of Neisseria gonorrhoeae and identification of other Neisseria species has been developed. This method utilizes both preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. Expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. The combined use of disposable glass tubes (6 by 50 mm) and microamounts of media (0.05 ml) make this method not only rapid, but also low in cost.

J Clin Microbiol. 1978 December; 8(6): 643-647






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