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J Clin Microbiol. 1979 May; 9(5): 645-647

Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae.

T S Walker and H H Winkler

ABSTRACT

A new assay is described for enumerating biologically active typhus rickettsiae (Madrid E strain), based on adsorption of rickettsiae to erythrocytes in the presence of NaF (which allows adsorption but not lysis) and lysis in the presence of anti-Rickettsia prowazeki immune serum (which allows only a single round of lysis). The number of lysed erythrocytes is then used to estimate the number of active rickettsiae.

J Clin Microbiol. 1979 May; 9(5): 645-647




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