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J. Clin. Microbiol. doi:10.1128/JCM.00654-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Two Year Study Evaluating the Potential Loss of Methicillin-Resistance in a MRSA Culture Collection

Center for Biological Defense, College of Public Health, University of South Florida, Tampa, Florida; Florida Department of Health, Bureau of Laboratories, Tampa, Florida

^* To whom correspondence should be addressed. Email: wveguill{at}health.usf.edu.

	Abstract

A reported loss of mecA prompted us to monitor 360 cryostocked MRSA strains for stability. Concurrently, 14 well-characterized strains were stored in the Microbank® preservation system and subjected to multiple freeze-thaw events. There were no significant declines in the methicillin-resistant populations with either method over a two year period.