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J. Clin. Microbiol. doi:10.1128/JCM.02083-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Inter- and Intra-assay Reproducibility of the Microplate Alamar Blue Assay for Isoniazid, Rifampicin, Ethambutol, Streptomycin, Ciprofloxacin, and Capreomycin Drug Susceptibility Testing of M. tuberculosis

Laboratorio de Investigación de Enfermedades Infecciosas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado No. 430, San Martín de Porras, Lima 31, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe St., Room 5515, Baltimore, MD 21205, USA; Asociación Benéfica PRISMA, Carlos Gonzales No. 251, San Miguel, Lima 32, Perú; Department of Infectious Diseases and Immunity and Wellcome Centre for Clinical Tropical Medicine, Imperial College London, Faculty of Medicine (Hammersmith Campus), DuCane Road, London W12 0NN, UK

	Abstract

The inter-sample and intra-sample variability of the microplate alamar blue assay (MABA) for indirect drug susceptibility testing of Mycobacterium tuberculosis was investigated. Between 1.2% and 8.5% of paired minimum inhibitory concentrations differed by >1 two-fold dilution, resulting in discordant susceptible/resistant designation at a frequency between 0.6% (rifampicin) and 18.9% (ethambutol).