This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Colombo, A. L.
Right arrow Articles by Rinaldi, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Colombo, A. L.
Right arrow Articles by Rinaldi, M. G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 03 1995, 535-540, Vol 33, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Comparison of Etest and National Committee for Clinical Laboratory Standards broth macrodilution method for azole antifungal susceptibility testing

AL Colombo, F Barchiesi, DA McGough and MG Rinaldi
Department of Pathology, University of Texas Health Science Center, San Antonio, USA.

The use of Etest strips for antimicrobial susceptibility testing is a new and promising method with broad applications in microbiology. Since these strips contain a predefined continuous gradient of a drug, it is possible to obtain a reproducible, quantitative MIC reading. We performed a prospective and double-blinded study to compare the Etest and National Committee for Clinical Laboratory Standards (Villanova, Pa.) broth macrodilution methods for determining the MICs of fluconazole, itraconazole, and ketoconazole for 100 clinical isolates (25 Candida albicans, 25 Cryptococcus neoformans var. neoformans, 20 Torulopsis [Candida] glabrata, 15 Candida tropicalis, and 15 Candida parapsilosis). The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-P guidelines. Despite differences between results for some species-drug combinations, Etest and macrobroth MICs were, in general, in good agreement. The MIC agreement rates for the two methods, within +/- 1 dilution, were 71% for ketoconazole, 80% for fluconazole, and 84% for itraconazole. According to our data, Etest has potential utility as an alternative method.


This article has been cited by other articles:

  • Dias, A. L. T., Matsumoto, F. E., Melhem, M. S. C., da Silva, E. G., Auler, M. E., de Siqueira, A. M., Paula, C. R. (2006). Comparative analysis of Etest and broth microdilution method (AFST-EUCAST) for trends in antifungal drug susceptibility testing of Brazilian Cryptococcus neoformans isolates.. J Med Microbiol 55: 1693-1699 [Abstract] [Full Text]  
  • Matar, M. J., Ostrosky-Zeichner, L., Paetznick, V. L., Rodriguez, J. R., Chen, E., Rex, J. H. (2003). Correlation between E-Test, Disk Diffusion, and Microdilution Methods for Antifungal Susceptibility Testing of Fluconazole and Voriconazole. Antimicrob. Agents Chemother. 47: 1647-1651 [Abstract] [Full Text]  
  • Maxwell, M. J., Messer, S. A., Hollis, R. J., Diekema, D. J., Pfaller, M. A. (2003). Evaluation of Etest Method for Determining Voriconazole and Amphotericin B MICs for 162 Clinical Isolates of Cryptococcus neoformans. J. Clin. Microbiol. 41: 97-99 [Abstract] [Full Text]  
  • Vandenbossche, I., Vaneechoutte, M., Vandevenne, M., De Baere, T., Verschraegen, G. (2002). Susceptibility Testing of Fluconazole by the NCCLS Broth Macrodilution Method, E-Test, and Disk Diffusion for Application in the Routine Laboratory. J. Clin. Microbiol. 40: 918-921 [Abstract] [Full Text]  
  • Rex, J. H., Pfaller, M. A., Walsh, T. J., Chaturvedi, V., Espinel-Ingroff, A., Ghannoum, M. A., Gosey, L. L., Odds, F. C., Rinaldi, M. G., Sheehan, D. J., Warnock, D. W. (2001). Antifungal Susceptibility Testing: Practical Aspects and Current Challenges. Clin. Microbiol. Rev. 14: 643-658 [Abstract] [Full Text]  
  • Osherov, N., Kontoyiannis, D. P., Romans, A., May, G. S. (2001). Resistance to itraconazole in Aspergillus nidulans and Aspergillus fumigatus is conferred by extra copies of the A. nidulans P-450 14{{alpha}}-demethylase gene, pdmA. J Antimicrob Chemother 48: 75-81 [Abstract] [Full Text]  
  • Arendrup, M., Lundgren, B., Jensen, I. M., Hansen, B. S., Frimodt-Moller, N. (2001). Comparison of Etest and a tablet diffusion test with the NCCLS broth microdilution method for fluconazole and amphotericin B susceptibility testing of Candida isolates. J Antimicrob Chemother 47: 521-526 [Abstract] [Full Text]  
  • Chang, H. C., Chang, J. J., Chan, S. H., Huang, A. H., Wu, T. L., Lin, M. C., Chang, T. C. (2001). Evaluation of Etest for Direct Antifungal Susceptibility Testing of Yeasts in Positive Blood Cultures. J. Clin. Microbiol. 39: 1328-1333 [Abstract] [Full Text]  
  • Kronvall, G., Karlsson, I. (2001). Fluconazole and Voriconazole Multidisk Testing of Candida Species for Disk Test Calibration and MIC Estimation. J. Clin. Microbiol. 39: 1422-1428 [Abstract] [Full Text]  
  • Aller, A. I., Martin-Mazuelos, E., Gutierrez, M. J., Bernal, S., Chavez, M., Recio, F. J. (2000). Comparison of the Etest and microdilution method for antifungal susceptibility testing of Cryptococcus neoformans to four antifungal agents. J Antimicrob Chemother 46: 997-1000 [Abstract] [Full Text]  
  • BOUCHARA, J.-P., ZOUHAIR, R., LE BOUDOUIL, S., RENIER, G., FILMON, R., CHABASSE, D., HALLET, J.-N., DEFONTAINE, A. (2000). In-vivo selection of an azole-resistant petite mutant of Candida glabrata. J Med Microbiol 49: 977-984 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Houston, A., Mills, K., Bolmstrom, A., Jones, R. N. (2000). Evaluation of the Etest Method for Determining Voriconazole Susceptibilities of 312 Clinical Isolates of Candida Species by Using Three Different Agar Media. J. Clin. Microbiol. 38: 3715-3717 [Abstract] [Full Text]  
  • Chang Chang, H., Jung Chang, J., Huey Huang, A., Chain Chang, T. (2000). Evaluation of a Capacitance Method for Direct Antifungal Susceptibility Testing of Yeasts in Positive Blood Cultures. J. Clin. Microbiol. 38: 971-976 [Abstract] [Full Text]  
  • Szekely, A., Johnson, E. M., Warnock, D. W. (1999). Comparison of E-Test and Broth Microdilution Methods for Antifungal Drug Susceptibility Testing of Molds. J. Clin. Microbiol. 37: 1480-1483 [Abstract] [Full Text]  
  • Martin-Mazuelos, E., Gutierrez, M. J., Aller, A. I., Bernal, S., Martinez, M. A., Montero, O., Quindos, G. (1999). A comparative evaluation of Etest and broth microdilution methods for fluconazole and itraconazole susceptibility testing of Candida spp.. J Antimicrob Chemother 43: 477-481 [Abstract] [Full Text]  
  • Espinel-Ingroff, A., Pfaller, M., Messer, S. A., Knapp, C. C., Killian, S., Norris, H. A., Ghannoum, M. A. (1999). Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candida spp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms. J. Clin. Microbiol. 37: 591-595 [Abstract] [Full Text]  
  • Witthuhn, F., Toubas, D., Béguinot, I., Aubert, D., Rouger, C., Remy, G., Pinon, J. M. (1999). Evaluation of the Fungitest Kit by Using Strains from Human Immunodeficiency Virus-Infected Patients: Study of Azole Drug Susceptibility. J. Clin. Microbiol. 37: 864-866 [Abstract] [Full Text]  
  • Sheehan, D. J., Hitchcock, C. A., Sibley, C. M. (1999). Current and Emerging Azole Antifungal Agents. Clin. Microbiol. Rev. 12: 40-79 [Abstract] [Full Text]  
  • Kirkpatrick, W. R., Turner, T. M., Fothergill, A. W., McCarthy, D. I., Redding, S. W., Rinaldi, M. G., Patterson, T. F. (1998). Fluconazole Disk Diffusion Susceptibility Testing of Candida Species. J. Clin. Microbiol. 36: 3429-3432 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Karlsson, A., Bolmström, A. (1998). Evaluation of the Etest Method for Determining Fluconazole Susceptibilities of 402 Clinical Yeast Isolates by Using Three Different Agar Media. J. Clin. Microbiol. 36: 2586-2589 [Abstract] [Full Text]  
  • Tortorano, A. M., Viviani, M. A., Barchiesi, F., Arzeni, D., Rigoni, A. L., Cogliati, M., Compagnucci, P., Scalise, G. (1998). Comparison of Three Methods for Testing Azole Susceptibilities of Candida albicans Strains Isolated Sequentially from Oral Cavities of AIDS Patients. J. Clin. Microbiol. 36: 1578-1583 [Abstract] [Full Text]  
  • Kirkpatrick, W. R., McAtee, R. K., Revankar, S. G., Fothergill, A. W., McCarthy, D. I., Rinaldi, M. G., Patterson, T. F. (1998). Comparative Evaluation of National Committee for Clinical Laboratory Standards Broth Macrodilution and Agar Dilution Screening Methods for Testing Fluconazole Susceptibility of Cryptococcus neoformans. J. Clin. Microbiol. 36: 1330-1332 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»