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Journal of Clinical Microbiology, January 1998, p. 100-104, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Abiotrophia elegans sp. nov., a Possible Pathogen in Patients with Culture-Negative Endocarditis

Andreas Roggenkamp,1,* Marianne Abele-Horn,1 Karl-Heinz Trebesius,1 Ursula Tretter,2 Ingo B. Autenrieth,1 and Jürgen Heesemann1

Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, 80336 Munich,1 and Krankenhaus Dritter Orden, 1 Medical Ward, 80638 Munich,2 Germany

Received 14 August 1997/Returned for modification 16 September 1997/Accepted 10 October 1997

We isolated a hitherto undescribed microorganism from a patient with endocarditis. The microscopic appearance, a negative catalase reaction, and growth as satellite colonies next to Staphylococcus epidermidis suggested that this microorganism is a member of the genus Abiotrophia, formerly known as nutritionally variant streptococci. However, the clinical isolate described herein differed markedly from the known Abiotrophia spp., A. adiacens and A. defectiva, in terms of its (i) biochemical properties, (ii) restricted growth temperature range, (iii) whole-cell lysate polypeptide profile, and (iv) unique nutritional requirements. In contrast to the type strains of A. adiacens and A. defectiva, which used L-cysteine and pyridoxal hydrochloride as growth factors, the growth of the clinical isolate was only supported by L-cysteine hydrochloride and not by pyridoxal hydrochloride when the organism was tested in Todd-Hewitt or casein-soy peptone broth. Comparative 16S rRNA gene sequence analysis revealed that the microorganism was a member of the genus Abiotrophia and was most closely related to A. adiacens (96.9% homology). Phenotypic and phylogenetic data are consistent with the assumption of a new species within the genus Abiotrophia, for which we propose the name Abiotrophia elegans sp. nov. The unique nutritional requirements of this strain are of importance for diagnostic laboratories. The media of blood culture systems supplemented only with pyridoxal hydrochloride as a growth factor may fail to promote the growth of A. elegans sp. nov., and thus, these systems might not detect this microorganism as a possible cause of endocarditis.

* Corresponding author. Mailing address: Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University Munich, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone: 49-89-51605200. Fax: 49-89-5380584. E-mail: Rogge{at}m3401.mpk.med.uni-muenchen.de.

Journal of Clinical Microbiology, January 1998, p. 100-104, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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