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Journal of Clinical Microbiology, October 2001, p. 3472-3480, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3472-3480.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Characterization of Cryptosporidium Isolates Obtained from Humans in France

K. Guyot,1,* A. Follet-Dumoulin,2 E. Lelièvre,1 C. Sarfati,3 M. Rabodonirina,4 G. Nevez,5 J. C. Cailliez,2 D. Camus,6 and E. Dei-Cas1,6

Ecologie du Parasitisme, Institut Pasteur de Lille, BP 245, 59019 Lille,1 Faculté Libre des Sciences, Institut Catholique de Lille, 59046 Lille,2 Parasitologie-Mycologie, Hôpital Saint-Louis, 75475 Paris,3 Parasitologie-Mycologie, Hôpital Edouard-Herriot, 69373 Lyon,4 Parasitologie-Mycologie, Centre Hospitalier Universitaire d'Amiens, Hôpital Sud, 80054 Amiens,5 and Parasitologie-Mycologie, Faculté de Médecine et Centre Hospitalier Régional et Universitaire de Lille, 59045 Lille,6 France

Received 7 May 2001/Returned for modification 21 June 2001/Accepted 18 July 2001

Cryptosporidium parvum is usually considered the agent of human cryptosporidiosis. However, only in the last few years, molecular biology-based methods have allowed the identification of Cryptosporidium species and genotypes, and only a few data are available from France. In the present work, we collected samples of whole feces from 57 patients from France (11 immunocompetent patients, 35 human immunodeficiency virus [HIV]-infected patients, 11 immunocompromised but non-HIV-infected patients) in whom Cryptosporidium oocysts were recognized by clinical laboratories. A fragment of the Cryptosporidium 18S rRNA gene encompassing the hypervariable region was amplified by PCR and sequenced. The results revealed that the majority of the patients were infected with cattle (29 of 57) or human (18 of 57) genotypes of Cryptosporidium parvum. However, a number of immunocompromised patients were infected with C. meleagridis (3 of 57), C. felis (6 of 57), or a new genotype of C. muris (1 of 57). This is the first report of the last three species of Cryptosporidium in humans in France. These results indicate that immunocompromised individuals are susceptible to a wide range of Cryptosporidium species and genotypes.

* Corresponding author. Mailing address: Ecologie du Parasitisme, Institut Pasteur de Lille, 1 rue du Pr. Calmette, BP 245, 59019 Lille, France. Phone: 33 3 20 87 71 56. Fax: 33 3 20 87 79 08. E-mail: karine.guyot{at}pasteur-lille.fr.

Journal of Clinical Microbiology, October 2001, p. 3472-3480, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3472-3480.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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