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Journal of Clinical Microbiology, June 2001, p. 2191-2196, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2191-2196.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genotyping Encephalitozoon hellem Isolates by Analysis of the Polar Tube Protein Gene

Lihua Xiao,^1,* Lixia Li,^1,3 Hercules Moura,^2,3 Irshad Sulaiman,¹ Altaf A. Lal,¹ Simonetta Gatti,⁴ Massimo Scaglia,⁴ Elizabeth S. Didier,⁵ and Govinda S. Visvesvara²

Immunology Branch¹ and Parasite Biology and Diagnostics Branch,² Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30341; Atlanta Research & Education Foundation, Atlanta, Georgia 30033,³ Laboratory of Clinical Parasitology, University of Pavia-IRCCS San Matteo, Pavla, Italy⁴; and Department of Microbiology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433⁵

Received 24 January 2001/Returned for modification 20 March 2001/Accepted 28 March 2001

To develop an alternative genotyping tool, the genetic diversity of Encephalitozoon hellem was examined at the polar tube protein (PTP) locus. Nucleotide sequence analysis of the PTP gene divided 24 E. hellem isolates into four genotypes, compared to two genotypes identified by analysis of the internal transcribed spacer of the rRNA gene. The four PTP genotypes differed from each other by the copy number of the 60-bp central repeat as well as by point mutations. A simple PCR test was developed to differentiate E. hellem genotypes based on the difference in the size of PTP PCR products, which should facilitate the genotyping of E. hellem in clinical samples.

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^* Corresponding author. Mailing address: Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Highway, Atlanta, GA 30341. Phone: (770) 488-4840. Fax: (770) 488-4454. E-mail: lax0{at}cdc.gov.

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Journal of Clinical Microbiology, June 2001, p. 2191-2196, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2191-2196.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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