This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, A.
Right arrow Articles by Bale, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walker, A.
Right arrow Articles by Bale, J. F., Jr.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2001, p. 2219-2226, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2219-2226.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Human Cytomegalovirus Strains by Analysis of Short Tandem Repeat Polymorphisms

Adrianne Walker,1 Susan J. Petheram,2 Linda Ballard,1 Jody R. Murph,3 Gail J. Demmler,4 and James F. Bale Jr.2,5,*

Departments of Pediatrics2 and Neurology,5 University of Utah School of Medicine,1 University of Utah, Salt Lake City, Utah; Department of Pediatrics, University of Iowa, Iowa City, Iowa3; and Department of Pediatrics, Baylor College of Medicine, Houston, Texas4

Received 28 November 2000/Returned for modification 16 January 2001/Accepted 12 March 2001

Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and found that a multiplexed PCR assay using primers based upon these STRs accurately maps HCMV strains. Using primers for 10 microsatellite regions, the STR profiles of 44 wild-type and 2 laboratory strains of HCMV were characterized. The results of STR analysis were compared with those for strain characterization using nucleotide sequencing and restriction fragment length polymorphism analysis. In each instance, STR analysis accurately and specifically identified strains that were indistinguishable or distinct by conventional molecular analysis. Analysis of short tandem repeats also detected polymorphisms that supported simultaneous excretion of two HCMV strains. These results indicate that STR analysis allows rapid, precise molecular characterization of HCMV strains.

* Corresponding author. Mailing address: Division of Pediatric Neurology, Suite 2700, Primary Children's Medical Center, 100 N. Medical Dr., Salt Lake City, UT 84113. Phone: (801) 588-3385. Fax: (801) 588-3392. E-mail: pcjbale{at}ihc.com.

Journal of Clinical Microbiology, June 2001, p. 2219-2226, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2219-2226.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Deback, C., Boutolleau, D., Depienne, C., Luyt, C. E., Bonnafous, P., Gautheret-Dejean, A., Garrigue, I., Agut, H. (2009). Utilization of Microsatellite Polymorphism for Differentiating Herpes Simplex Virus Type 1 Strains. J. Clin. Microbiol. 47: 533-540 [Abstract] [Full Text]  
  • Pasqualotto, A. C., Denning, D. W., Anderson, M. J. (2007). A Cautionary Tale: Lack of Consistency in Allele Sizes between Two Laboratories for a Published Multilocus Microsatellite Typing System. J. Clin. Microbiol. 45: 522-528 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»